Method for the producing vanillic acid and vanillin from...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S136000, C435S147000, C435S254100, C435S254300

Reexamination Certificate

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07462470

ABSTRACT:
A method for the production of vanillic acid and vanillin from waste residue (niger) of rice bran oil by microbial fermentation and biotransformation is provided. The method of the present invention comprises the steps of hydrolization of ferulic acid esters in waste residue of rice bran oil to ferulic acid and further conversion into vanillic acid using microbial strainAspergillus nigerCGMCC 0774 screened and preserved by the present inventor, followed by conversion of vanillic acid to produce vanillin using another microbial strainPycnoporus cinnabarinusCGMCC 1115. The method for the production of vanillin according to the present invention utilizes renewable source from rice processing and alleviates the environmental pollution caused by the chemical synthesis of vanillin.

REFERENCES:
patent: 5866380 (1999-02-01), Lesage-Meessen et al.
Stentelaire et al.; Design of a Fungal Bioprocess for Vanillin Production from Vanillic Acid at Scalable Level byPycnoporus cinnabarinus; Journal of Bioscience and Bioengineering; vol. 89, No. 3, 223-230; 2000.
In the May 2000 edition of “Chemistry in Britain”, pp. 48-50; Chemistry in Britain, 2000; 36 (5).

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