Method for the prevention of hemoglobin interference in reagent

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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436 63, 436 66, 436 91, 436 98, 436135, 436166, 436169, 436175, 422 56, 435 4, 435 28, G01N 3348, G01N 33493, G01N 3370, C12Q 128

Patent

active

060748818

ABSTRACT:
The present invention involves an improvement to an assay for the activity of a metal chelate, such as copper II/creatinine, which activity is related to the concentration of the analyte in a fluid test sample. In carrying out the assay, there is combined the fluid test sample, the metal chelate or precursors thereof, a hydroperoxide, an oxidizable indicator and a pyrazole derivative which inhibits the ability of hemoglobin to oxidize the oxidizable dye.

REFERENCES:
patent: 4350762 (1982-09-01), De Luca et al.
patent: 4637978 (1987-01-01), Dappen
patent: 5183741 (1993-02-01), Arai et al.
patent: 5374561 (1994-12-01), Pugia
patent: 5418142 (1995-05-01), Kiser et al.
patent: 5702955 (1997-12-01), Pugia

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