Method for the preparation of yeast chromatin for isolation of p

Details Method for the preparation of yeast chromatin for isolation of p Method for the preparation of yeast chromatin for isolation of p

1985-07-11

1988-08-30

Rosen, Sam

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435 68, 530824, C07K 312

Patent

active

047677147

ABSTRACT:
A method for the preparation of pure yeast chromatin for isolation of p20 polypeptide includes breaking yeast cells in a buffer solution and separating the released chromatin. The chromatin is purified through sucrose, solubilized by an enzymatic digestion and the supernatant solution is loaded on a linear sucrose gradient 5-30% in a buffer solution. The p20 polypeptide is extracted from the sucrose gradient fraction by a buffer solution comprising sodium chloride at a concentration in the range of 0.15-5 M and preferably in the range of 0.35 M to 2.0 M.

REFERENCES:
patent: 3867255 (1975-02-01), Newell et al.
patent: 3960659 (1976-06-01), Fazakerley
patent: 3996104 (1976-12-01), Gunilla et al.
Gulloev et al-Chem. Abst. vol. 94 (1981), p. 61282f.

Affiliated with

Also associated with

Rating

Method for the preparation of yeast chromatin for isolation of p does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the preparation of yeast chromatin for isolation of p, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the preparation of yeast chromatin for isolation of p will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2088067