Method for the preparation of transgenic plants...

Details Method for the preparation of transgenic plants... Method for the preparation of transgenic plants...

2004-05-19

2010-06-08

Kubelik, Anne (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Nonplant protein is expressed from the polynucleotide

C800S278000, C800S279000

Reexamination Certificate

active

07732665

ABSTRACT:
The present invention relates to a method for the preparation of transgenic plants, tissues or plant cells thereof having long-lasting resistant to the geminiviruses by a) “identification” or “selection” of a viral gene sequence encoding an amino acid sequence able to confer resistance against geminiviruses; b) mutagenesis or “choice” of the viral gene sequence so as to make it an ineffective target of the post-trascriptional silencing induced by infecting geminivirus; and c) insertion of the geminivirus mutated or chosen gene sequence obtained in step b) through a construct as described previously, in the plant, tissue or plant cell thereof.

REFERENCES:
patent: 2005/0125862 (2005-06-01), Polston et al.
patent: WO 00/43520 (2000-07-01), None
van Wezel et al 2002, MPMI 15:203-208.
Vanderschuren et al, 2007 Plant Biotechnology Journal 207-220.
Lazar et al. 1988, Mol. Cell. Biol. 8:1247-1252.
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Guo et al. 2004, Proc. Natl. Acad. Sci. USA 101: 9205-9210.
Brunetti Angela et al:, “Transgenically expressed T-Rep of tamato yellow leaf curl sardinia virus acts as a trans-dominant-negative mutant, inhibiting viral transcription and replication”, Journal of Virology, vol. 75, No. 22, Nov. 2001, pp. 10573-10581, XP002300782, ISSN: 0022-538X, the whole document.
Noris E et al:, Resistance to tomato yellow leaf curl geminivirus in Nicotiana benthamiana plants transformed with a truncated viral C1 gene, Virology, Academic Press, Orlando, US, vol. 224, No. 1, 1996, pp. 130-138, XP002142359, ISSN: 0042-6822, the whole document.

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