Method for the preparation of spherical microorganism cell aggre

Details Method for the preparation of spherical microorganism cell aggre Method for the preparation of spherical microorganism cell aggre

1983-02-24

1985-09-24

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435177, 435182, 435233, C12N 1102, C12N 1108, C12N 1104, C12N 990

Patent

active

045433321

ABSTRACT:
Spherically shaped bacterial cell aggregates are produced by spheronizing extruded flocculated cells. The cells are flocculated from aqueous medium with a cross-linked polyamine which is the reaction product of an epihalohydrin/polyamine copolymer and a cross-linking agent. Prior to being extruded, a filter cake having 68 to 76 weight percent water is produced by filtration of the flocculated cells, and the filter cake is ground into particles no greater than 60 mesh. Spheronizing is with a plate rotating at a tangential velocity of 4.5 to 12 meters per second within a cylinder containing the plate. Toughness of the spherical aggregates produced can be increased by the addition of a binder after filtration and before extrusion. During spheronizing, fines may be produced. These fines can be recycled by mixing them with the wet filter cake and binder before extrusion.

REFERENCES:
patent: 4212943 (1980-07-01), Borglum
patent: 4251632 (1981-02-01), Chen et al.
Conine, et al., Drug and Cosmetic Industry, Apr. 1970.
Manufacturing Chemist and Aerosol News, Jun. 1970.

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