Method for the preparation of prostaglandin synthetase

Details Method for the preparation of prostaglandin synthetase Method for the preparation of prostaglandin synthetase

1977-03-23

1978-01-24

Shapiro, Lionel M.

Textiles: fiber preparation

C07G 7026

Patent

active

040702421

ABSTRACT:
A method for the preparation of purified prostaglandin synthetase from bovine seminal vesicles is disclosed. The method includes the steps of homogenizing bovine seminal vesicles with a liquid medium to form an homogenate and centrifuging the homogenate at a speed such that the centrifugal force does not exceed 1,000g. The supernatant obtained, containing prostaglandin synthetase, is separated from the solids.

REFERENCES:
takeguchi et al. in Biochemistry, vol. 10, pp. 2372-2376 (1971).
Flower et al. in Prostaglandins, vol. 4, No. 3, pp. 325-341 (1973).
Yoshimoto et al. in Journal of Biochemistry, vol. 68, pp. 487-499 (1970).

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