Method for the preparation of natural human growth hormone in pu

Details Method for the preparation of natural human growth hormone in pu Method for the preparation of natural human growth hormone in pu

1988-12-22

1991-09-10

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 694, 435 697, 43525231, 530399, 935 47, 935 48, 935 74, C12P 2106, C12P 2102, C12N 1518, C07K 1300

Patent

active

050473336

ABSTRACT:
An improved method is described for the preparation of natural human growth hormone (hGH) in pure form, which comprises the preparation of a precursor of hGH by means of the culture of Bacillus subtilis cells transformed by a hybrid plasmid, the separation of the precursor from the total proteins, the digestion of the precursor with the enzyme Factor Xa and finally the purification of the natural hGH thus obtained from the enzymatic hydrolysis mixture.

REFERENCES:
patent: 4532207 (1985-07-01), Brewer et al.
patent: 4601980 (1986-07-01), Goeddel et al.
Dodet, B., 1990, Biofutur, Jun. 1990, pp. 20-29.
Nagai et al, Proc. Nat'l. Academy of Sci. (U.S.A.), vol. 82, pp. 7252-7255 (1985).

Affiliated with

Also associated with

Rating

Method for the preparation of natural human growth hormone in pu does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the preparation of natural human growth hormone in pu, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the preparation of natural human growth hormone in pu will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-539520