Method for the preparation of multiple glucosyl branched-cyclode

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 96, 435 97, 435 98, 536 41, 536103, 536123, C12P, C12P 1918, C07G 300, C08B 3716

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049313894

ABSTRACT:
In a method for preparing multiple glucosyl branched-cyclodextrins, a debranching enzyme is contacted to a mixture of maltose or a substance containing maltooligosaccharide such as maltose with a cyclodextrin to obtain a reaction product from which a branched-cyclodextrin is then separated; said branched-cyclodextrin is converted to glycosyl-cyclodextrin under the action of glucoamylase, which is thereafter mixed with maltose or a substance containing maltooligosaccharide such as maltose; and the resulting mixture is successively acted on by a debranching enzyme and glucoamylase.

REFERENCES:
patent: 4668626 (1987-05-01), Kobayashi et al.
Handbook of Amylases and Related Enzymes, p. 125, (1988).
Chemical Abstracts, 107(18):156851s, (1987).

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