Method for the preparation of human growth hormone

Details Method for the preparation of human growth hormone Method for the preparation of human growth hormone

1992-02-12

1993-12-07

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525231, 4353201, C12P 2102, C12N 1575, C12N 1500

Patent

active

052682773

ABSTRACT:
A hybrid plasmid which is capable of inducing the expression and the secretion of human growth hormone (hGH) in its natural form and a method for the preparation of hGH which comprises the culture in a suitable environment of Bacillus subtilis cells transformed by the hybrid plasmid, are described.
The hGH thus obtained has the correct amino acid sequence and is particularly useful for treatment of man.

REFERENCES:
Sigma Chemical Company Biochemicals, Organic Compounds for Research and Diagnostic Reagents, 1992, p. 1329.
Nakayama et al; J. Biotechnology 5: 171 (1987).
Vasantha et al; Gene 49: 23 (1986).
Stahl et al; J. Bacteriology 158: 411 (1984).

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