Method for the preparation of gliadin- and glutenin-rich...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...

Reexamination Certificate

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C530S370000, C530S372000, C530S412000, C530S418000, C530S427000

Reexamination Certificate

active

07385037

ABSTRACT:
Method for the preparation of gliadin- and glutenin-rich fractions from gluten in an aqueous medium and in the presence of an acid, wherein the gluten are dispersed continuously or not in water up to a dry substance varying between 5 and 30%, by which the pH of the dispersion is monitored between 4.4 and 4.8 and the gluten-water mixture is submitted to shearing actions, through which the dispersion, continuously or not, can be fractionated in gliadin- and glutenin-rich fractions, by which a single gliadin-rich fraction with a gliadin/glutenin ratio of at least 2.5 is obtained, and a single glutenin-rich fraction with a gliadin/glutenin ratio of less than 0.8 is obtained.

REFERENCES:
patent: 5610277 (1997-03-01), Bassi et al.
patent: 685164 (1995-12-01), None
patent: 992193 (2000-04-01), None
patent: 09/169798 (1997-06-01), None
patent: 09/176192 (1997-07-01), None
patent: WO 97/10260 (1997-03-01), None
Berot et al.;Pilot scale preparation of wheat gluten protein fractions I-Influence of process parameters on their protein composition: International Journal of Food Science and Technology, 1994; vol. 29; pp. 489-502.
Goforth & Finney;Separation of Glutenin from Gliadin by Ultracentrifugation; Communication to the Editor, Ceareal Chemistry; 1976; Amer. Assn. of Cereal Chemists; vol. 53, No. 4: pp. 608-612.

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