Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1985-02-04
1987-05-26
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 96, 435 98, 435 97, C12P 1918, C12P 1922, C12P 1920, C12P 1916
Patent
active
046686266
ABSTRACT:
The invention provides an efficient enzymatic method for the preparation of branched cyclodextrins such as glucosyl and maltosyl cyclodextrins. The inventive method comprises the enzymatic reaction of a branch-splitting enzyme and .beta.-amylase simultaneously with a mixture of a cyclodextrin and starch. Alternatively, a branched cyclodextrin is obtained from a mixture of a cyclodextrin and maltose in an enzymatic reaction with pullulanase, optionally, with admixture of an alcohol such as ethyl and propyl alcohols or a glycol such as ethyleneglycol and propyleneglycol to the reaction mixture. A glucosyl cyclodextrin can be obtained by the steps of first subjecting a mixture of a cyclodextrin and maltose to an enzymatic reaction with pullulanase and then subjecting the reaction product to a second enzymatic reaction in the presence of an enzyme mixture composed of takaamylase and glucoamylase and yeast.
REFERENCES:
patent: 4384898 (1983-05-01), Okada et al.
Chemical Abstracts, vol. 98, 3491v.
Nature, vol. 210, p. 200 (Apr. 9, 1966).
Kainuma Keiji
Kobayashi Shoichi
Director of National Food Research Institute, Ministry of Agricu
Shapiro Lionel M.
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