Method for the preparation of bacterial clones carrying optimal

Chemistry: molecular biology and microbiology – Vector – per se

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435 68, 435 70, 4351723, 4353171, 435 91, 536 27, 935 9, 935 29, 935 38, 935 57, 935 60, 935 72, C12P 1934, C12P 2100, C07H 1512, C12N 1500

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active

048574708

ABSTRACT:
The DNA coding for human recombinant growth hormone releasing factor (GRF) cloned into a bacterial plasmid expression vector by assembling in one step selected synthetic DNA fragments.

REFERENCES:
patent: 4517181 (1985-05-01), Ling et al.
Barr et al. (1983), Fed. Proceedings, vol. 42, Abst. #1160, p. 1956.
Spiess et al., (1982), Biochemistry, vol. 80, pp. 5037-6040.
Gubler et al., Proc. Nat'l Acad. Sci. USA, vol. 80, pp. 4311-4314.
Soll et al., eds (1982), The Applications of Computers to Research on Nucleic Acids, IRL Press, Oxford and Washington, D.C., Narang, (1983), Tetrahedron, 39:3-22.

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