Method for the preparation of an immobilized protein ultrathin f

Details Method for the preparation of an immobilized protein ultrathin f Method for the preparation of an immobilized protein ultrathin f

1996-10-16

2000-08-22

Riley, Jezia

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

356244, 427 213, 427414, 435 71, 4352991, 4352879, 436518, 436810, C12M 100, G01N 104, G01N 3353

Patent

active

06107084&

ABSTRACT:
A method for the preparation of an immobilized protein ultrathin film reactor by immersing a solid support alternately into an aqueous solution of protein, and into an aqueous solution of polyion charged oppositely to said protein and preparing a structurally controlled ultrathin film of mono- or multi- protein layers on a said solid support with precision at molecular level. And a method for chemical reaction of a substrate by preparing an immobilized protein ultrathin film reactor composed of multiple layers of protein on a solid support by above mentioned method, and initiating a chemical change of the substrate molecules using obtained immobilized protein ultrathin film reactor.

REFERENCES:
patent: 5063081 (1991-11-01), Cozzette et al.
patent: 5521702 (1996-05-01), Salamon et al.
patent: 5643721 (1997-07-01), Spring et al.
Lvov et al. "Assembly of multicomponent protein films by means of electrostatic layer-by-layer adsorption" JACS, vol. 117, No. 22, pp. 6117-6123, 1995.
Lvov et al. "Layer-by-layer assembly of alternate protein/polyion ultrathin films" Chemistry Letters, No. 12, pp. 2323-2326, 1994.

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