Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1987-11-30
1991-04-23
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 6951, 435 697, 435 698, 435 772, 4351723, 43525231, 4353201, 435231, 935 48, 935 74, C12P 2100, C12N 1500, C12N 120, C12N 986
Patent
active
050100009
ABSTRACT:
A selected protein or protein part is prepared in Bacillus strain bacteria by joining DNA encoding the selected protein or protein part essential to its biological activity to a bacterium gene. The Bacillus amyloliquefaciens gene for .alpha.-amylase is cleaved at a location after the excretion signal following the regulation sequence, or at a location after a part essential with respect to its excretion. The cleaved gene is joined to a plasmid present in Bacillus strain bacteria in several copies, and the DNA sequence encoding the selected protein or protein part essential to its biological activity is joined to the cleavage site by recombinant DNA techniques. Bacillus strain host bacteria are transformed with the recombinant DNA molecules so obtained, and the transformed host bacteria cultivated to produce the selected protein or protein part.
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Genesit Oy
Martinell James
Oy Alko AB
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