Method for the preparation of a phospholipid-dependent prothromb

Details Method for the preparation of a phospholipid-dependent prothromb Method for the preparation of a phospholipid-dependent prothromb

1992-11-30

1995-09-26

Knode, Marian C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 424542, C12N 950, C12N 948

Patent

active

054533708

ABSTRACT:
A method for the preparation of a phospholipid-dependent prothrombin activator from the venom of snakes belonging to the Elapidae family, the activator comprising an increased plasma clotting activity in the presence of phospholipids and calcium ions; a reduced activity in the presence of Lupus Anticoagulant; an ability to facilitate clotting in a normal clotting time in the presence of platelet poor plasma, the plasma having normal or decreased levels of factor V, VII, VIII, IX, or X; and a major band with a molecular weight of 40,000 to 60,000 daltons on an SDS gel.

REFERENCES:
patent: 4106992 (1978-08-01), Vairel et al.
patent: 4137127 (1979-01-01), Stocker
patent: 4610879 (1986-09-01), Markland, Jr. et al.
patent: 4725673 (1988-02-01), Herring
patent: 5112949 (1992-05-01), Vukovich
patent: 5192689 (1993-03-01), Hemker et al.
patent: 5219995 (1993-06-01), Herring et al.
patent: 5260060 (1993-11-01), Markland, Jr. et al.
Owen, W. G. et al., "Trombosis Research". vol. 3(6), 1973, pp. 705-714.
Pirkle, H. et al, "Thrombosis Research," vol. 1(6), 1972, pp. 559-568.
Govers-Rienslag, J. W. P. et al., "Haenostasis and Animal Verams," Hematology, vol. 7, 1988., pp. 41-53. editor--H. Pirkle et al., Marcel-Dekker, Inc., New York, N.Y.
Speijer, H. et al, "Thrombosis and Haemostesis" vol. 57(3), 1987 (Jun.), pp. 349-355.
Owen, W. G., et al., "Thromb. Haemostasis," vol. 42(1), 1979 Jul., p. 400, #0954-abstract.
Whitaker, A. N. et al., "Aust N. Z. J. Med., " vol. 18(3, suppl. #2), 1988, p. 430, abstract.
Chester, A., et al., "Toxicon," vol. 20(2), 1982, pp. 501-504.
Lalloo, D., et al., "Toxicon," vol. 30(5-6), 1992, p. 528.
Speijer, H., et al., "The J. of Biological Chem.," vol. 261(28), Oct. 5, 1986, pp. 13258-13267.
Masci, P. P., et al., "Thrombosis Research," vol. 59, 1990, pp. 859-870.
Scopes, R. K., Protein Purification--Principles and Practice, 2nd ed., Springer-Volag, 1988, pp. 1, 17-20, 41-42, 50-54.
Deutscher, M. P., ed., Methods in Enzymology, vol. 182-Guide to Protein Purification, Academic Press, Inc., 1990, pp. 285, 292-295.
Fohlman, J., "Toxicon," vol. 17, 1979, pp. 170-172.
Tibballs, J., "Anaesth. Inters. Care," vol. 20(1), Feb. 1992, pp. 28-32.
Walker, F. J., et al., "Biochemistry," vol. 19, 1980, pp. 1020-1023.
Nakagaki, T., et al., "Thrombosis Research," vol. 65, 1992, pp. 105-116.
D. A. Triplett et al., "Lupus Anticoagulants: Misnomer, Paradox, Riddle Epiphenomenon," Hematol. pathol. 2, 121-143, 1988.
P. E. Love et al., "Antiphospholipid Antibodies: Anticardiolipin and the Lupus Anticoagulant in Systemic Lupus Erythematosus (SLE) and in Non-SLE Disorders," Ann. Int. Med. 112, 682-698, 1990.
T. Exner et al., SSC Subcommittee for the Standardization of Lupus Anticoagulants, "Guidelines for Testing and Revised Criteria for Lupus Anticoagulants," Thromb. Haemost. 65, 320-322, 1991.
J. Rosing et al., "Inventory of Exogenous Prothrombin Activators," Thrombosis and Haemostasis 65, 627-630, 1991.
P. P. Masci et al., "Purification and Characterization of a Prothrombin Activator from the Venom of the Australian Brown Snake, Pseudonaja Textilis Textilis," Biochemistry International, 17, 825-835, 1988.
C. Y. Lee (ed.), Snake Venoms, pp. 15-40, Springer Verlag, Berlin, Heidelberg, New York (1979).
H. Hofmann et al., "Blood Coagulation Induced by the Venom of Bothrops atrox. 1. Identification, Purification, and Properties of a Prothrombin Activator," Biochemistry, 26, 772-780 (1987).
T. Morita et al., "Purification and Properties of Prothrombin Activator from the Venom of Echis carinatus", J. Biochem. 83, 559-570 (1978).

Affiliated with

Also associated with

Rating

Method for the preparation of a phospholipid-dependent prothromb does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the preparation of a phospholipid-dependent prothromb, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the preparation of a phospholipid-dependent prothromb will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1551558