Method for the preparation of a non-woven silk fibroin fabrics

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Scleroproteins – e.g. – fibroin – elastin – silk – etc.

Reexamination Certificate

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C530S355000, C514S012200, C435S007100

Reexamination Certificate

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10398252

ABSTRACT:
A process for producing non-woven silk fiber fabrics comprises the following steps: a) obtaining silk fibroin, for example either from silk cocoons, or silk textiles or waste silk; b) removing the sericin layer covering the silk fibroin fibers, when present; c) breaking the disulfide bonds between heavy (350 kDa) and light (27 kDa) chains of silk fibroin in order to obtain the production of chain fragments which serve as a specific cellular recognition sites promoting the attachment and growth of cells, d) homogenising of the material resulting from step c).

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Raje et al. ,Man-Made Textiles in India 41(4), 160-167 (1998).
N. Minoura, M. Tsukada, M. Nagura, “Physico-chemical properties of silk fibroin membrane as a biomaterial” Biomaterials,11, pp. 430-434, 1990 (On Order).
M. Demura, T.Asakura ,T. Kuroo, “Immobilization of biocatalyst with Bombix mori silk fibroin by several kinds of physical treatment and its application to glucose sensors”, Biosensors, pp. 361-372, 1989 (On Order).
T. Hanawa, A. Wanabe, T. Tsuchiya, R. Ikoma, M. Hidika, M. Sugihara, “New oral dosage form for elderly patients: Preparation and characterization of silk fibroin gel”, Chem. Pharm. Bull. 43, 284-288, 1995 (On Order).

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