Method for the preparation of a labelled virus without the inact

Details Method for the preparation of a labelled virus without the inact Method for the preparation of a labelled virus without the inact

1990-08-02

1992-12-01

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

422 58, 435 30, 435 34, 435 39, 435296, 436536, 436538, 436805, 436824, C17Q 100

Patent

active

051680378

ABSTRACT:
A method for the preparation of a labelled target-specific virus where the binding sites on the virus are not inactivated by the label material. And a three-step process for detection of a targeted microorganism, the first step comprising contacting of labelled target-specific virus with a sample suspected of containing a target microorganism, the second step comprising the capture of the labelled virus/microorganism complexes onto a solid matrix, and the third step comprising the detection of said complexes on the solid matrix. The process is highly sensitive and can be used for rapid detection of target microorganisms in clinical, food, pharmaceutical, cosmetic and environmental samples. The invention further concerns a device and a kit based on the inventive process.

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Stanier et al., "The Microbial World", 5th Edition, (Prentice Hall, New Jersey), 1986, pp. 214-217.
"Detection of Food Pathogens Using the Phage Rapid Identification Assay System," a poster session presentation at the Institute of Food Technologists Annual Meeting in Jun. 1987.

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