Method for the particularly sensitive detection of nucleic acids

Details Method for the particularly sensitive detection of nucleic acids Method for the particularly sensitive detection of nucleic acids

1995-11-22

1998-07-21

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 935 77, 935 78, C12Q 168, C12N 1500, C12P 1934

Patent

active

057833923

ABSTRACT:
A method of detecting a target nucleic acid A is disclosed, comprising hybridizing the target nucleic acid A with a probe nucleic acid B which contains a sequence B1 which base pairs with a part of the target nucleic acid A and a sequence B2, cleaving the hybridized probe nucleic acid B to produce a cleavage product B' containing the sequence B2, hybridizing the cleavage product B' with a template nucleic acid C containing a sequence C2 which base pairs with a part of the cleavage product B' and a sequence C1 which does not hybridize with the sequence B1 of the probe nucleic acid B, extending the hybridized cleavage product B' with an extension sequence B3 which is template-specific to a part of the sequence C1, hybridizing a probe D with the extension product, wherein the probe D contains a sequence D1 which base pairs with the extension sequence B3 and a sequence D2, and detecting any of the various products formed throughout the method. Products for performing the method are also disclosed.

REFERENCES:
patent: 4994368 (1991-02-01), Goodman et al.
patent: 5011769 (1991-04-01), Duck et al.

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