Method for the particularly sensitive detection of nucleic acids

Details Method for the particularly sensitive detection of nucleic acids Method for the particularly sensitive detection of nucleic acids

1998-02-09

1999-12-14

Zitomer, Stephanie

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435875, 536 243, 536 2433, 536 231, 935 77, 935 78, C12P 1934, C12Q 168, C07H 2102, C07H 2104

Patent

active

060016108

ABSTRACT:
A method of detecting a target nucleic acid A is disclosed, comprising hybridizing the target nucleic acid A with a probe nucleic acid B which contains a sequence B1 which base pairs with a part of the target nucleic acid A and a sequence B2, cleaving the hybridized probe nucleic acid B to produce a cleavage product B' containing the sequence B2, hybridizing the cleavage product B' with a template nucleic acid C containing a sequence C2 which base pairs with a part of the cleavage product B' and a sequence C1 which does not hybridize with the sequence B1 of the probe nucleic acid B, extending the hybridized cleavage product B' with an extension sequence B3 which is template-specific to a part of the sequence C1, hybridizing a probe D with the extension product, wherein the probe D contains a sequence D1 which base pairs with the extension sequence B3 and a sequence D2, and detecting any of the various products formed throughout the method. Products for performing the method are also disclosed.

REFERENCES:
patent: 4945368 (1990-07-01), Goodman et al.
patent: 5011769 (1991-04-01), Duck et al.
patent: 5200313 (1993-04-01), Carrico
patent: 5736365 (1998-04-01), Walker et al.
patent: 5744308 (1998-04-01), Guillou-Bonnici et al.
patent: 5744311 (1998-04-01), Fraiser et al.
patent: 5824517 (1998-10-01), Cleuziat et al.

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