Method for the non-radioactive measurement of the nucleic acid s

Details Method for the non-radioactive measurement of the nucleic acid s Method for the non-radioactive measurement of the nucleic acid s

1994-04-19

1995-10-03

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 436 63, 436 94, 436501, 436829, 436 231, 935 76, 935 77, 935 78, 264 41, 264 43, C12Q 168, G01N 3353, C07H 2104, C07H 2102

Patent

active

054551579

ABSTRACT:
For the determination of the rate of nucleic acid synthesis in eukaryotic cells a nucleotide which is non-radioactively labelled or derivatized with a hapten is introduced into the cells with the addition of liposomes and the rate of synthesis of the nucleic acids is determined by means of the incorporation of the nucleotide via its label or derivatization.

REFERENCES:
patent: 4515736 (1985-05-01), Deamer
patent: 4585736 (1986-04-01), Dolbeare et al.
patent: 4711955 (1987-12-01), Ward et al.
Porstmann et al., J. Immunol. Meth., 82: 169-179 (1985).
Felgner et al., Proc. Natl. Acad. Sci. 84: 7413-7417 (Nov. 1987).
Stamatatos et al., Biochem. 27: 3917-3925 (1988).

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