Method for the multiplexed preparation of nucleic acid molecular

Details Method for the multiplexed preparation of nucleic acid molecular Method for the multiplexed preparation of nucleic acid molecular

1994-12-05

1998-02-03

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 231, 536 243, 536 2432, C12Q 168, C12P 1934, C07H 2104, C07H 2102

Patent

active

057143269

ABSTRACT:
A method of making a pool of individual DNA molecular weight markers of defined base pair lengths that are resolved from one another and have substantially equal band intensities and that span the desired range of base pair lengths; the resultant pool of DNA molecular weight markers; the method of determining the presence of a nucleic acid or the length thereof in a specimen suspected of containing a nucleic acid utilizing such resultant pool of markers, and kits utilizing the method of making the pool and/or utilizing the markers.

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patent: 5316908 (1994-05-01), Carlson et al.
Hartley et al. Nucleotide sequence of the yeast plasmid. Gene (1980) 286:860-865.
Perkin Elmer Cetus, GeneAmp PCR Core Reagents 1991.
Perkin Elmer Cetus, GeneAmp DNA Amplification Reagent Kit 1991.
Yonemura et al. A New Assay Method for DNase by Fluorescence Polarization and Fluorescence . . . J. Biochem. (1982) 92:1297-1303.
Courtens et al. Nucleus of the boar spermatozooa, structure and modifications in frozen, frozen-thawed . . . Mol. Reprod. Dev. (1989) 1:264-277. Abstract.
1989 Bethesda Research Catalogue & Reference Guide, (1989) Life Technologies, Inc., pp. 76-84.
Tung et al. "PCR Amplif. of Specific Seq. from a cDNA Library" in: Erlich, PCR Technology (New York, Stockton Press, 1989, pp. 99-104.
Saiki et al. "The Design and Optimization of the PCR" in: Erlich, PCR Technology (New York, Stockton Press, 1989, pp. 7-16.

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