Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-07-20
1995-10-03
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 74, 435 79, 435 792, 435 4, 435 29, 435 39, 435183, 435 18, G01N 33574, G01N 33573, G01N 3353
Patent
active
054551617
ABSTRACT:
The invention relates to a method for assessing clinical activity and resistance to multiple anticancer drugs of mammalian cells, based on a dose-dependent inhibition with cytotoxic agents of the cellular rise in ornithine decarboxylase activity, following serum stimulation, which comprises the steps of: (a) determining by an immunological technique the rise in the ornithine decarboxylase protein levels, after adding serum containing medium to quiescent mammalian cells, and (b) obtaining the levels of the anticancer drug resistance from the ratio of 50% inhibitory concentration of ornithine decarboxylase obtained with an anticancer drug for drug resistant cells and drug sensitive cells. The immunological technique is selected from ELISA, radioimmunoassay, immunofluorescence, flow cytometry and fluorescence microscopy. The increase in the ornithine decarboxylase activity results from an increase in the number of ornithine decarboxylase molecules.
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Assaraf Yehuda G.
Bachrach Uriel
Scheiner Toni R.
Technicon Reseach & Development Foundation Ltd.
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