Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-03-05
1988-12-20
Warren, Charles F.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435243, 935 78, C12Q 168, C12N 100
Patent
active
047925191
ABSTRACT:
A method is disclosed for the monitoring and control of a microbial population in a biotechnological system. The method involves the identification of at least one critical subpopulation and determining the optimal level for said subpopulation. A controlled variable is determined which can be adjusted to alter the level of the subpopulation. The method employs nucleic acid hybridization in the microbial population. This involves the preparation of a labelled probe from nucleic acid having a nucleotide sequence substantially complementary to a nucleotide sequence in the nucleic acid in the subpopulation. A representative sample of the microbial population is obtained and treated to free nucleic acids and to denature double-stranded nucleic acids. The resulting sample nucleic acids are contacted with the labelled probe under appropriate conditions to form duplexes. The labelled probe in the duplex is monitored to determine the amount of duplexes and the level of the subpopulation is calculated from the determined duplexes. The controlled variable is adjusted based on the calculated level of the subpopulation to bring the level toward the optimal level. The method is used to monitor and control the microbial population without reliance upon the phenotypical expression of the genetic information of the organisms.
REFERENCES:
patent: 4064015 (1977-12-01), Nyiri et al.
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4480040 (1984-10-01), Owens
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4593000 (1986-06-01), Sumino et al.
Sayler, G. S. et al, App. Environ. Micro., vol. 49, May 1985, pp. 1295-1303.
Shields, M. S., J. Micro. Meth., vol. 6, 1986, pp. 33-46.
Moseley et al. (1982), Identification of Enterotoxigenic E. Coli by Colony Hybridization Using Three Enterotoxin Gene Probes, J. of Inf. Dis., 145(6):863-869.
Fitts (1985), Development of a DNA-DNA Hybridization Test for the Presence of Salmonella, in Foods, Food Technol., 39(3):95-102.
Klausner et al. (1983), Gene Detection Technology Opens Doors for Many Industries, Biotechnology (Aug.):471-478.
Regulating Biotechnology, Thomas O. McAarity--Issues in Science & Technology, Spring 1985, pp. 40-56.
Ecological Consequences: Reducing the Uncertainities, Martin Alexander, Issues in Science & Technology--Spring 1985, pp. 57-68.
Our Investment: What is at Stake?, Ralph W. F. Hardy & David J. Glass, Spring, 1985, pp. 69-52.
Biodegradation: Its Measurement and Microbiological Basis, C. P. Leslie Grady, Jr., Biotechnology & Bioengineering, vol. 27, May, 1985, pp. 660-674.
Groundwater Pollution Microbiology, Franklin R. Leach, Groundwater Pollution Microbiology, John Wiley & Sons, .COPYRGT.1984, pp. 304-351.
Dynamics of Continuous Commensalistic Cultures, Satish J. Parulekar & Henry C. Lim, Paper presented at the AIChE 1984 Annual Meeting, San Francisco, CA.
Comparative Effects of Aroclor 1254 (Polychlorinated Biphenyls) and Phenanthrene on Glucose Uptake by Freshwater Microbial Populations, G. S. Sayler, L. C. Lund, et al, Applied & Environmental Microbiology, May, 1979, pp. 878-885.
Stability of Colicin Plasmids in Continuous Culture: Mathematical Model & Analysis, Douglas A. Lauffenburger, Biotechnology Progress (vol. 1, No. 1), Mar. 1985, pp. 53-59.
Heterotrophic Bacterial Guild Structure: Relationship to Biodegradative Populations, Lawrence M. Mallory and Gary S. Sayler, Microbial Ecology (1983), 9:41-55.
Colony Hybridization: A Method for the Isolation of Cloned DNAs that Contain a Specific Gene, Michael Grunstein and David S. Hogness, Proc. Natl. Acad. Sci. (USA), vol. 72, No. 10, pp. 3961-3965, Oct. 1975.
An Improved Colony Hybridization Method with Significantly Increased Sensitivity for Detection of Single Genes, Renata Maas, Plasmid 10, 296-298 (1983), .COPYRGT.1983 by Academic Press, Inc.
Rapid Procedure for Detection and Isolation of Large and Small Plasmids, C. I. Kado and S. T. Liu, Journal of Bacteriology, Mar. 1981, pp. 1365-1373.
Plasmid Screening at High Colony Density, Douglas Hanahan & Matthew Meselson, Gene, 10 (1980), 63-67.
DNA-DNA Hybridization Assay for Detection of Salmonella spp. in Foods, Renee Fitts et al, Applied & Environmental Microbiology, Nov. 1983, pp. 1146-1151, vol. 46, No. 5.
Foodborne Enterotoxigenic Escherichia Coli: Detection and Enumeration by DNA Colony Hybridization, Walter E. Hill et al, Applied & Environmental Microbiology, Apr. 1983, pp. 1324-1330, vol. 45, No. 4.
Detection and Enumeration of Virulent Yersinia Enterocolitica in Food by DNA Colony Hybridization, Walter E. Hill et al, Applied & Environmental Microbiology, Sep. 1983, pp. 636-641, vol. 46, No. 3.
Medical Microbiology--Detection of Enterotoxigenic Escherichia Coli by DNA Colony Hybridization, S. L. Moseley et al, The Journal of Infectious Diseases, vol. 142, No. 6, Dec. 1980, pp. 892-898.
A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA, H. C. Birnboim and J. Doly, Nucleic Acids Research, vol. 7, No. 6, 1979, pp. 1513-1523.
A Colorimetric Method for DNA Hybridization, Manfred Renz and Christina Kurz, Nucleic Acids Research, vol. 12, No. 8, 1984, pp. 3435-3444.
Rapid and Sensitive Colorimetric Method for Visualizing Biotin-Labeled DNA Probes Hybridized to DNA or RNA Immobilized on Nitrocellulose: Bio-Blots, Jeffry J. Leary et al, Proc. Natl. Acad. Sci USA, vol. 80, pp. 4045-4049, Jul. 1983.
Molecular Characterization of Environmental and Nontoxigenic Strains of Vibrio Cholerae, James B. Kasper et al, Infection & Immunity, May, 1981, pp. 661-667.
Molecular and Functional Analysis of the TOL Plasmid pWWO from Pseudomonas Putida and Cloning of Genes for the Entire Regulated Aromatic Ring Meta Cleavage Pathway, F. C. H. Franklin et al, Proc. Natl. Acad. Sci USA, vol. 78, No. 12, pp. 7458-7462, Dec. 1981, Biochemistry.
Genetic Rearrangements in Plasmids Specifying Total Degradation of Chlorinated Benzoic Acids, Deb. K. Chatterjee and A. M. Chakrabarty, Mol. Gen. Genet (1982), 188: 279-285.
Involvement of Plasmids in Total Degradation of Chlorinated Biphenyls, Applied & Environmental Microbiology, Sep. 1982, pp. 619-626.
Molecular Relationships Between Pseudomonas INC P-9, Degradative Plasmids TOL, NAH and SAL, Philip R. Lehrbach et al, Plasmid, 10, 164-174 (1983).
Isolation of a Mutant TOL Plasmid with Increased Activity and Transmissibility from Pseudomonas Putida (Arvilla) mt-2, Journal of Bacteriology, Jan. 1977, pp. 39-46, vol. 129, No. 1.
Plasmid Gene Organization: Naphthalene/Salicylate Oxidation, K. M. Yen and I. C. Gunsalus, Proc. Natl. Acad. Sci. USA, vol. 79, pp. 874.varies.878, Feb. 1982, Genetics.
Cloning of Genes for Naphthalene Metabolism in Pseudomanas Putida, Alan D. Grund and I. C. Gunsalus, Journal of Bactibiology, Oct. 1981, pp. 89-94, vol. 150, No. 1.
Genetic Aspects of Biodegradation by Pseudomonads, D. Haas, Experienta, 39 (1983), pp. 1195-1213.
Blackburn James W.
Sayler Gary S.
International Technology Corporation
Jay Jeremy M.
University of Tennessee Research Corporation
Warren Charles F.
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