Method for the microbiological isomerisation of...

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Resolution of optical isomers or purification of organic...

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C435S252900, C435S136000

Reexamination Certificate

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07829324

ABSTRACT:
The present invention relates to a method for the microbiological isomerization of alpha-hydroxycarboxylic acids using an alpha-hydroxycarboxylic acid racemase, the enzymes used for this method and microorganisms which express a suitable racemase activity, a screening method for microorganisms with alpha-hydroxycarboxylic acid racemase activity, the nucleic acid sequences encoding this enzyme, expression vectors, recombinant microorganisms which express this racemase, and methods for the production or isolation of a protein with alpha-hydroxycarboxylic acid racemase activity.

REFERENCES:
patent: 5534436 (1996-07-01), Seufer-Wasserthal et al.
patent: 6605430 (2003-08-01), Affholter et al.
patent: 0596466 (1994-05-01), None
Dennis et al., Annals of the New York Academy of Sciences, Jul. 1965, vol. 119, No. 3 : pp. 868-876.
Hiyama et al. , J Biochem.1968; 64: 99-107.
Stetter et al., Arch Mikrobiol. Dec. 31, 1973;94(3):221-47.
DSMZ Catalogue on line, accessed Jun. 26, 2008 (1 page)—http://www.dsmz.de/microorganisms/html/strains/strain.dsm020017.html.
DSMZ Catalogue on line, accessed Jun. 26, 2008 (1 page)—http://www.dsmz.de/microorganisms/html/strains/strain.dsm020207.html.
Glueck, S. M., et al., “Lactate Racemase As a Versatile Tool for the Racemization of α-Hydroxycarboxylic Acids”, Chem. Listy, 2003, vol. 97, p. 431.
Schnell, B., et al., “Enzymatic Racemisation and its Application to Synthetic Biotransformations”, Advanced Synthesis & Catalysis, 2003, vol. 345, pp. 653-666.
Liu, S-Q., “Practical Implications of Lactate and Pyruvate Metabolism by Lactic Acid Bacteria in Food and Beverage Fermentations”, International Journal of Food Microbiology, 2003, vol. 83, pp. 115-131.
Strauss. U.T., et al., “Deracemization of (±)-Mandelic Acid Using a Lipase-Mandelate Racemase Two-enzyme System”, Tetrahedron:Asymmetry, 1999, vol. 10, pp. 4079-4081.
Felfer, U., et al., “Substrate Spectrum of Mandelate Racemase Part 2. (Hetero)-Aryl-Substituted Mandelate Derivatives and Modulation of Activity”, Journal of Molecular Catalysis B: Enzymatic, 2001, vol. 15, pp. 213-222.
Schafer, S.L., et al., “Mechanism of the Reaction Catalyzed by Mandelate Racemase: Structure and Mechanistic Properties of the D270N Mutant”, Biochemistry, 1996, vol. 35, pp. 5662-5669.
Garcia-Viloca, M., et al., “A QM/MM Study of the Racemization of Vinylglycolate Catalyzed by Mandelate Racemase Enzyme”, Journal of the American Chemical Society, 2001, vol. 123, pp. 709-721.
Li, R., et al., “Racemization of Vinylglycolate Catalyzed by Mandelate Racemase”, Journal of Organic Chemistry, 1995, vol. 60, pp. 3347-3351.
Kenyon, G.L., et al., “Mandelate Racemase: Structure—Function Studies of a Pseudosymmetric Enzyme”, Acc. Chem. Res., 1995, vol. 28, pp. 178-186.
Pearson, W.R., et al., “Improved Tools for Biological Sequence Comparison”, Proc. Natl. Acad. Sci. USA, 1988, vol. 85, pp. 2444-2448.
Narang, S.A., “DNA Synthesis”,Tetrahedron, 1983, vol. 39, No. 1, pp. 3-22.
Itakura, K., et al., “Synthesis and Use of Synthetic Oligonucleotides”, Ann. Rev. Biochem., 1984, vol. 53, pp. 323-356.
Itakura, K., et al., Expression inEscherichia coliof a Chemically Synthesized Gene for the Hormone Somatostatin, 1977, vol. 198, pp. 1056-1063.
Ike, Y., et al., “Solid Phase Synthesis of Polynucleotides. VIII. Synthesis of Mixed Oligodeoxyribonucleotides by the Phosphotriester Solid Phase Method”, Nucleic Acids Research, 1983, vol. 11, No. 2, pp. 477-488.
Arkin, A.P., et al., “An Algorithm for Protein Engineering: Simulations of Recursive Ensemble Mutagenesis”, Proc. Natl. Acad. Sci. USA, 1992, vol. 89, pp. 7811-7815.
Delagrave, S., et al., “Recursive Ensemble Mutagenesis”, Protein Engineering, 1993, vol. 6, No. 3, pp. 327-331.
Thomas, K.R., et al., “Site-Directed Mutagenesis by Gene Targeting in Mouse Embryo-Derived Stem Cells”, Cell, 1987, vol. 51, pp. 503-512.
Ebbers, E.J., et al., “Controlled Racemization of Optically Active Organic Compounds: Prospects for Asymmetric Transformation”,Tetrahedron, 1997, vol. 53, No. 28, pp. 9417-9476.
van Rantwijk, F., et al., “Lipase-Catalyzed Synthesis of Carboxylic Amides: Nitrogen Nucleophiles as Acyl Acceptor”, Monatshefte für Chemie, 2000, vol. 131, pp. 549-569.
Stockland, A. E., et al., “Multiple Forms of Lactate Dehydrogenase inStaphylococcus aureus”, Journal of Bacteriology, 1969, vol. 100, No. 1, pp. 347-353.
Hino, T., et al,, “Presence of Lactate Dehydrogenase and Lactate Racemase inMegasphaera elsdeniiGrown on Glucose or Lactate”, Applied and Environmental Microbiology, 1993, vol. 58, No. 1, pp. 255-259.
Pepple, J. S., et al., “Lactate Racemase: Hydroxylamine-Dependent18O Exchange of the α-Hydroxyl of Lactic Acid”, Biochimica et Biophysica Acta, 1976, vol. 429, pp. 1036-1040.

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