Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-01-31
1997-05-20
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 793, 435 794, 435 795, 435961, 435973, 436518, 436531, 436543, 436544, 436811, 436822, G01N 3353
Patent
active
056311389
ABSTRACT:
A method for the measurement of serum bile acids by ELISA comprising: preparing a bile acid active ester, reacting the ester with a bovine serum albumin solution, dialyzing, and immunizing a mammal other than human being with the dialyzate thus obtained as an antigen to thereby give an anti-bile acid antibody; reacting said active ester with an enzyme to thereby prepare an enzyme-labeled bile acid as an enzyme-labeled antigen; to a secondary antibody-coated plate, adding a dilution of the serum to be assayed, an anti-bile acid antibody solution and an enzyme-labeled antigen solution and reacting these substances followed by the addition of a substrate and the reaction therewith; measuring the absorbance of the reaction mixture and determining the concentration of each bile acid on the basis of the standard curve measured simultaneously; and referring the sum of the concentrations of these bile acids to the total bile acid concentration; and a method for the diagnosis of a liver disease comprising: calculating the concentration ratio of each bile acid from the values measured by the former method; and comparing the concentration ratio thus obtained with the standard level scope of said concentration ratio obtained from healthy subjects are provided.
REFERENCES:
patent: 4256724 (1981-03-01), Rutner et al.
patent: 4410634 (1983-10-01), Cooper et al.
patent: 4623485 (1986-11-01), Kono et al.
patent: 5141865 (1992-08-01), Croze et al.
Beckett et al, "Investigations Into the Choice of Immunogen, Ligand, Antiserum, and Assay Conditions for the Radioimmunoassay of Conjugated Cholic Acid", Clinica Chemica Acta 88(1978)257-266.
Demers et al., "RIA of Bile Acids in Serum," Clin. Chem. 22(1976):602-606.
Maentausta et al, "RIA of Conjugated Cholic Acid, Chenodeoxycholic Acid, and Deoxycholic Acid from Human Serum . . . " Clin. Chem. 25(1979):264-268.
Ross, "Radioimmunoassay of Serum Bile Acids," Methods in Enzymology 84(1982):321-349.
Chemical Abstract 91(19):153824 "Enzyme-labeled Immunoassay for a Bile Acid in Serum" (1979).
Chemical Abstract 90:164295 "Enzyme-linked Immunoassay of Ursodeoxycholic Acid in Serum" (1979).
Patent Abstracts of Japan 4(51):53-93628 "Measuring Method for Bile Acid in Blood Using .sup.125 I Marked Combination Bile Acid Tyrosin Methyl Ester Derivative" (1980).
C. Paul Bianchi, Chemical Abstracts, Jun. 1976, vol. 84, No. 25 abstract No. 178092.
C. Paul Bianchi, Chemical Abstracts, Nov. 1980, vol. 93, No. 21 abstract No. 200494.
J. Morikawa et al., Patent Abstracts of Japan, Apr. 1980, vol. 4, No. 51, abstract #55-20453.
Rose et al., "Enzyme-Linked Immunosorbent Assay", Manual of Clinical Laboratory Immunology, Third Edition, Chapter 17 (1986) pp. 99-109.
Kano M., Wada H., Matsumoto M., Yamamoto K., Kamano T., Motegi K., Oguchi K. and Kano Y.: Human fecal bile acids and fractions with enzyme-linked immunosorbent assay, Showa Univ. J. Med. Sci., 5: 183 - 191 (1993).
Reddy B.S. and Wynder E.L.: Metabolic epidemiology of colon cancer, Fecal bile acid and neutral sterols in colon cancer patients and patients with adenomatous polyps, Cancer, 39: 2533 - 2539 (1977).
Kumada T., Nakano S., Ohta H., Sasaki T., Kitamura K., Watahiki H., Takeda I., Okuyama S. and Takagi K.; Clinical evaluation on determination of individual bile acids in liver diseases, serum bile acid composition in fulminant hepatitis and acute hepatitis, Kanzhs, 23: 364 - 371 (1982) (English abstract).
Karlaganis G. and Paumgartner G.: Determination of bil acids in serum by capillary gas-liquid chromatography, Clin. Chim. Acta, 92: 19 - 26 (1979).
Maruyama K., Tanimura H. and Hikasa Y.: Analysis of conjugated bile acids in bile by high pressure liquid chromatography, Clin. Chim. Acta, 100: 47 - 54 (1980).
Mashige F., Tanaka N., Maki A., Kamei S. and Yamanaka M.: Direct spectrophotometry of total bile acids in serum, Clinchem., 27: 1352 - 1356 (1981).
Inaguma H., Katagiri K., Tsukada K., Hirose A., Hoshino M., Hayakawa T., Yokochi M., Miyaji M., Ito M. and Takeuchi T.: Serum bile acid composition in fulminant hepatitis, comparative study with acute hepatitis, Nagoya Med. J., 29: 63 - 71 (1984).
Abe K., Yoshiba M. and Sugata F.: Clinical significance of kinetics of serum bile acid fractions in patients with fulminant hepatitis and liver cirrhosis, J. Showa Med. Assoc., 53: 25 - 30 (1993) (English abstract).
Ayaki Y. and Yamasaki K.: In vitro conversion of 7.alpha.-hydroxycholesterol to some natural C.sub.24 -bile acis with special reference to chenodeoxycholic acid biogenesis, J. Biochem., 68: 341 - 346 (1970).
Kano Motonari
Matsumoto Masaru
Spiegel Carol A.
Yuugengaisha B.S.R.
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