Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-03-08
1996-04-16
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C12Q 168
Patent
active
055081665
ABSTRACT:
A method is provided for the determination of the presence of a reverse transcriptase in a sample liquid. In an aqueous medium, at least an adenine ribopolynucleotide RNA template (poly A), an immobilized oligodeoxythyminenucleotide and a biotinylated deoxyuridine triphosphate are reacted in the presence of the sample liquid. After separation of the reacted and the unreacted biotinylated deoxyuridine triphosphate from each other, the reacted or the unreacted biotinylated deoxyuridine triphosphate is detected. Also provided is a method for the determination of infection of a patient to a retrovirus. The reaction is conducted in the presence of a sample body fluid and the reverse transcriptase. After the detection of the reacted or the unreacted biotinylated deoxyuridine triphosphate, the reverse-transcriptase-neutralizing and/or inhibiting antibody titer in the sample body fluid is determined.
REFERENCES:
patent: 3755086 (1973-08-01), Heimer
patent: 4851331 (1989-07-01), Vary et al.
patent: 4942122 (1990-07-01), Imagawa et al.
Langer, P. R., et al., Enzymatic synthesis of biotih-labeled polynucleotides: Novel nucletic acid affinity probes. Proc. Natl. Acad. Sci. U.S.A. (Nov. 1981) 78:6633-6637.
Sano, K., et al., Antibody that inhibits human immunodeficiency virus reverse transcriptase and association . . . J. Cl. z. Microbiol. (Dec. 1987) 25:2415-2417.
Lund, V., et al., Assesment of methods for covalent binding of nucleic acids to magnetic beads, Dynabeads.TM., and the . . . Nucl. Acids. Res. (1988) 16:10861-10880.
Proc. Natl. Acad. Sci. vol. 78, No. 11, pp. 6633-6637, Nov. 1981 "Enzymatic synthesis of biotin-labeled . . . ".
Nature vol. 226 Jun. 27, 1970 pp. 1209-1211 "Viral RNA-dependent DNA Polymerase".
Nature vol. 226 Jun. 27, 1970 pp. 1211-1213.
Journal of Virological Methods, vol. 27, pp. 269-276, 1990, "A Solid Phase Reverse Transcriptase Micro-Assay For The Detection Of Human Immunodeficiency Virus And Other Retroviruses In Cell Culture Supernatants", P. A. Somogyi, et al.
Mizoguchi Junzo
Nakai Masuyo
Sano Kouichi
Tanno Masashi
Asahi Kasei Kogyo Kabushiki Kaisha
Elliott George C.
LandOfFree
Method for the measurement of reverse transcriptase using immobi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the measurement of reverse transcriptase using immobi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the measurement of reverse transcriptase using immobi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-324393