Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1988-01-05
1991-09-10
Nucker, Christine
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 25, 435 28, 435805, 436175, 422 56, 422 57, 422 58, C12Q 134
Patent
active
050473298
ABSTRACT:
A method for measuring creatine in a sample by the use of creatine amidinohydrolase which comprises decomposing the N-ethylglycine present in the sample enzymatically and thereafter reacting sarcosine oxidase upon the sample; and a reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 20 mM or below and catalase or comprises said sarcosine oxidase, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, a sarcosin oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 50 mM or above, peroxidase and a color reagent for H.sub.2 O.sub.2.
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Noda Institute for Scientific Research
Nucker Christine
Scheiner Laurie
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