Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2008-04-01
2008-04-01
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S006120, C435S069700, C435S069900
Reexamination Certificate
active
07351549
ABSTRACT:
The present invention relates to a method for the manufacture and purification of recombinant trypsinogen and trypsin inE. coliand yeast, using high yield expression vectors with and without secretion leader sequences. The invention further relates to an improved method and apparatus for carrying out protein refolding specifically useful for processing trypsinogen that has accumulated intracellularly in the form of inclusion bodies.
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patent: WO 97/00316 (1997-01-01), None
patent: WO 99/18219 (1999-04-01), None
Emi, M. et al, Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogen. Gene 41: 305-310 (1986).
Bouffard, G.G. et al. A Collection of 1814 Human Chromosome 7-Specific STSs. Genome Research 7: 59-64 (1997).
Brodrick, J.W. et al. Human Cationic Trypsinogen: Purification, Characterization, and Characteristics of Autoactivation. The Journal of Biological Chemistry 253(8): 2732-2736 (1978).
Kay, J., et al., “The Autoactivation of Trypsinogen”, The Journal of Biological Chemistry, vol. 246, No. 21, 1971, pp. 6661-6665, XP-001030933.
Sahin-Toth, M., et al., “High-Affinity CA2+Binding Inhibits Autoactivation of Rat Trypsinogen”, Biochemical and Biophysical Research Communications, vol. 275, No. 2, Aug. 28, 2000, pp. 668-671, XP-002181528.
Hohenblum Hubertus
Katinger Hermann
Mattanovich Diethard
Naschberger Stefan
Weik Robert
Guzo David
Joike Michele K.
Polymun Scientific Immunbiologische Forschung GmbH
Rothwell Figg Ernst & Manbeck P.C.
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