Method for the isolation of symbiotic microorganisms from host p

Details Method for the isolation of symbiotic microorganisms from host p Method for the isolation of symbiotic microorganisms from host p

1980-12-03

1982-12-28

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435803, C12Q 124

Patent

active

043662455

ABSTRACT:
Symbiotic microorganisms such as nitrogen fixing bacteria and phosphorus-providing fungi are isolated from nodules and mycorrhiza on the roots of host plants by sterilizing the surface and subsurface areas of root tissue with an aqueous solution of a fixative containing osmium tetraoxide. The osmium tetraoxide is highly soluble in water, and volatilizes at room temperature to form a vapor which penetrates subsurface air pockets in root tissues to kill contaminating microorganisms. The osmium tetraoxide also provides a visual indicator of its progress through plant tissue by a progressive darkening of such tissue from the surface of root samples toward their centers. In this manner, the sterilization can be halted before the centrally located causitive bacteria is destroyed.

REFERENCES:
Dale Callaham et al., Science, vol. 199, pp. 899-902 (1978).
"The Isolation and Cultivation of Actinomycetous Root Nodule Endophytes", In Symbiotic Nitrogen Fixation in the Management of Temperate Forests, Oregon St. University, proceedings of Workshop held, Apr. 2-5, 1979, pp. 38-56, Baker et al.
Baker et al., "Separation of Actinomycete Nodule Endophytes from Crushed Nodules Suspensions by Sephadex Fractionation," Bot. Goz., vol. 140 (suppl.), 549-551 (1979).

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