Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-07
1998-07-21
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4241301, 435 11, 435962, 436175, 436178, 436501, 436518, 436536, 436824, 436825, C12Q 160, G01N 33573
Patent
active
057834008
ABSTRACT:
A method is provided for preparing lipoprotein (a) from a volume of biological fluid that is substantially free of lipoproteins of another class. The method involves an ultracentrifugation step in which at least one fraction is recovered that contains Lp(a). This material is then reacted with immobilized ligand to remove non-Lp(a) interfering substances from the fraction, the Lp(a) remaining unbound. The non-reacted Lp(a) is subsequently obtained in a form that is suitable for use in the analysis of any of protein concentration, protein isoform determination or cholesterol assays. A method of identifying and measuring an amount of one or more isoforms of Lp(a) is further provided.
REFERENCES:
patent: 4244694 (1981-01-01), Farina et al.
patent: 4366244 (1982-12-01), Pascal
patent: 4746605 (1988-05-01), Kerscher et al.
patent: 4945040 (1990-07-01), Fless et al.
patent: 5229073 (1993-07-01), Luo et al.
patent: 5320968 (1994-06-01), Seman
patent: 5403745 (1995-04-01), Ollington et al.
Medline Abstract No. 93043129., 1992.
Berg, Acta Pathol. Microbiol. Scand., 59:369-382., 1963.
Schaefer et al., NEJM 312:1300-1310., 1985.
Gaubatz, et al., Methods in Enzymology 129:167-186., 1986.
McNamara et al., Clinica Chimica Acta 166:1-8., 1987.
N.I.H. Publication No. 88-2925., 1988.
Utermann, Hum. Genet. 78:41-46., 1988.
Gaubatz, J. Lipid Res. 31:603-613., 1990.
Craig, Appl. Theor. Electrophoresis 2:135-140., 1991.
Marcovina, Biochem. Biophys. Res. Comm. 191:1192-1196.,1993.
Karmansky, Clin. Biochem. 27:151-162., 1994.
Leerink, Fibrinolysis 8:214-220., 1994.
Seman et al., Clin. Chem. 40:400-403., 1994.
Kulkarni et al., J. Lipid Research 35:159-168., 1994.
Campos et al., Clinica Chimica Acta 230:43-50., 1994.
Nauck et al., Clin. Chem. 41:731-738., 1995.
Christopher C. William
Gebski Christine P.
Ollington James F.
Genzyme Corporation
Housel James C.
Swartz Rodney P.
LandOfFree
Method for the isolation of lipoprotein allowing for the subsequ does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the isolation of lipoprotein allowing for the subsequ, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the isolation of lipoprotein allowing for the subsequ will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1645930