Method for the isolation of hybridomas using cholesterol auxotro

Details Method for the isolation of hybridomas using cholesterol auxotro Method for the isolation of hybridomas using cholesterol auxotro

1989-07-31

1992-05-05

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524031, 4352402, 435 702, 4352403, 43524021, C12N 500

Patent

active

051107376

ABSTRACT:
Cholesterol auxotrophy of myeloma cells is used as the basis for selecting hybridomas. The outgrowth of nascent hybridomas in a cholesterol-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma cells. This method of hybridoma selection can be applied with any sterol-dependent parent cell line. The nutrient medium is also preferably free of Ham's F-12 nutrient mixture.

REFERENCES:
patent: 2787581 (1957-04-01), Scherr
Myoken et al, "A New Method for the Isolation of NS-1 Hybridomas Using Cholesterol Auxotrophs of NS-1 Myeloma Cells", Fourth International Cell Biology Congress, Montreal, Canada, Aug. 14-19, 1988.
Leonard et al, Biochem. Biophys. Acta 947:101-112 (1988).
Perlman, D., Meth. Enzymol. 58:110-116 (1979).
Sigma Catalog, 1991, p. 169.

Affiliated with

Also associated with

Rating

Method for the isolation of hybridomas using cholesterol auxotro does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the isolation of hybridomas using cholesterol auxotro, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the isolation of hybridomas using cholesterol auxotro will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1412020