Method for the isolation and purification of hepatitis B surface

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...

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424 89, 530380, 530415, 530806, 530826, 530808, A61K 3929, C07K 318, C07K 328

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046491929

ABSTRACT:
The supernatant of engineered yeast cells having produced hepatitis B surface antigaen and disrupted in the presence of a polysorbate non-ionic detergent is clarified by addition of urea and adsorbed on colloidal silica from which the purified antigen is desorbed with a low ionic strength buffer supplemented with urea and a polysorbate non-ionic detergent.

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Hitzeman et al., Nucl. Acids Res. 11(9):2745 (1983).

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