Method for the isolation and presumptive diagnosis of Bacillus c

Details Method for the isolation and presumptive diagnosis of Bacillus c Method for the isolation and presumptive diagnosis of Bacillus c

1982-08-09

1984-04-24

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435834, C12Q 104, C12R 1085

Patent

active

044448830

ABSTRACT:
A method is provided for the isolation and presumptive diagnosis of Bacillus cereus which method is useful in the assay of antibiotic preparations, food products such as milk, drug products and cosmetics. A medium for use in such method is also provided which medium includes tellurite, glycine and polymyxin, and inhibits most organisms which grow and resemble Bacillus cereus on this medium. If an organism grows on the medium and has characteristic colonies, a presumptive diagnosis of Bacillus cereus can be assumed until further tests are made.

REFERENCES:
patent: 4308346 (1981-12-01), Newano
Bergey's Manual of Derminative Bacteriology, 8th Ed., pp. 534-535, 1974.
S. Finrgold et al., Bailey and Scott's Diagnostic Microbiology, 5th Ed., pp. 20 and 21; 1978.
Donovan, K. O., "A Selective Medium for Bacillus cereus in Milk," J. Appl. Bacteriol., 21:100-103 (1958).
Kim et al., "Occurrence of Bacillus cereus in Selected Dry Food Products," J. Milk Food Technol. 34:12-15 (1971).
Kim et al., "Enumeration and Identification of Bacillus cereus in Foods," Appl. Microbiol., 22:581-587 (1971).
Mossel, et al., "Enumeration of Bacillus cereus in Foods," Appl. Microbiol., 15:650-653 (1967).
Fleming, A. F., "On the Specific Antibacterial Properties of Penicillin and Potassium Tellurite," J. Pathogenic. Bacteriol., 35:831-842 (1932).
Zebovitz et al., "Tellurite-Glycine Agar:A Selective Plating Medium for the Quantitative Detection of Coagulase-Positive Staphylococci," J. Bacteriol., 70:686-690 (1955).

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