Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1991-01-02
1999-11-23
Sisson, Bradley
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435196, C12N 922, C12N 914
Patent
active
059898860
ABSTRACT:
A new type of dominant negative gene-fusion acts as an antiviral agent when expressed in infected cells. The dominant negative effect is obtained by fusing a gene coding for a capsid or envelope protein of a virus to a gene coding for an enzyme (e.g., nuclease or protease) that can destructively hydrolyze or modify the encapsulated viral DNA, RNA or proteins. The fusion gene is expressed in virally infected cells; viruses assembled in these cells will then "self-destruct" because the fusion enzyme incorporated in the viral particles destroys or functionally alters essential components of the virus, thus preventing the virus from productively infecting another cell.
REFERENCES:
patent: 4593002 (1986-06-01), Dulbecco
patent: 4769330 (1988-09-01), Paoletti et al.
patent: 4774182 (1988-09-01), Szybalski
patent: 4918166 (1990-04-01), Kingsman et al.
patent: 5008373 (1991-04-01), Kingsman et al.
patent: 5041379 (1991-08-01), Fraser et al.
Baltimore, Cell, vol. 40, pp. 481-482, 1985.
Baltimore, Nature, vol. 335, pp. 395-396, 1988.
Boeke, Mobile DNA: Transposable Elements in Saccharomyces cerevisiae, eds. Douglas E. Berg and Martha M. Howe, Am. Soc. for Microbiology, Wash., D.C., Chapter 13, pp. 335-374, 1989.
Boeke, et al., Mol. and Cell. Biol., vol. 8, No. 4, pp. 1432-1442, 1988.
Boeke, et al., Cell, vol. 40, pp. 491-500.
Boeke, et al., Science, 239, pp. 280-282, 1988.
Chejanovsky, et al., J. Virology, vol. 64, pp. 1764-1770, 1990.
Eichinger, et al., Cell, vol. 54, pp. 955-966, 1988.
Friedman, et al., Nature, vol. 335, pp. 452-454, 1988.
Garfinkel, et al., Cell, vol. 42, pp. 507-517, 1985.
Garry, et al., Science, vol. 250, pp. 1127-1129, 1990.
Gilmour, et al., AIDS, vol. 3, pp. 717-723, 1989.
Green, et al., Cell, vol. 58, pp. 215-223, 1989.
Hartley, J. Mol. Biol., vol. 202, pp. 913-915, 1988.
Herskowitz, Nature, vol. 329, pp. 219-222, 1987.
Jones, et al., J. Virology, vol. 64, No. 5, pp. 2265-2279, 1990.
Kingsman, et al., Trans. R. Soc. Lond., vol. 324, pp. 477-485, 1985.
Boeke, "Yeast Ty Elements as Retroviruses" in Viruses of Fungi and Simple Eukaryotes, ed. Koltin & Leibowitz, Marcel Dekker, NY, 1988.
Malim, et al., Cell, vol. 58, pp. 205-214, 1989.
Mariani, et al., Nature, vol. 347, pp. 737-741, 1990.
Mossakowska, et al., Biochemistry, vol. 28, pp. 3843-3850, 1989.
Rose, et al., Cell, vol. 48, pp. 1047-1060, 1987.
Salter, et al., Virology, vol. 157, pp. 236-240, 1987.
Shortle, Gene, vol. 22, pp. 181-189, 1983.
Towbin, et al., Proc. Natl. Acad. Sci. USA, vol. 76, No. 9, pp. 4350-4354, 1979.
Trono, et al., Cell, vol. 59, pp. 113-120, 1989.
Varmus, Nature, vol. 314, pp. 583-584, 1985.
Weldon, et al., J. Virology, vol. 64, pp. 4169-4179, 1990.
Boeke, et al., Int'l Search Report of PCT/US92/00014, filed Jan. 2, 1992.
Boeke Jef D
Natsoulis Georges
Bugaisky G. E.
Sisson Bradley
The Johns Hopkins University
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