Method for the inactivation of amylase in the presence of...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Enzyme inactivation by chemical treatment

Reexamination Certificate

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Reexamination Certificate

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07955829

ABSTRACT:
The invention relates to a method for the inactivation of amylase in a solution comprisingRhizomucor mieheiaspartic protease by keeping the solution at a pH between 2.1 and 2.8 and at a temperature between 20° C. and 40° C. for a period of time sufficient to inactivate amylase for at least 95%. With this method the level of residualRhizomucor mieheiaspartic protease activity in the solution at the end of the heating time is at least 90%, preferably at least 95%, and the residual amylase activity is 0.05 RAU/g or lower, more preferably 0.001 RAU/g or lower, i.e. more than 99% of the amylase activity has been inactivated.

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International Search Report for PCT/EP02/14529, mailed on Aug. 1, 2003, 4 pages.
Sternberg, J. Dairy Sci. (1971) 2(54):159-167.

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