Method for the in vitro titration of an NCTA application...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007210, C435S007920, C436S501000, C436S518000, C422S067000

Reexamination Certificate

active

07727728

ABSTRACT:
The invention relates to a method for the in vitro titration of a non-conventional transmissible agent (NCTA), using transgenic cell lines. The invention also relates to: the application of the aforementioned in vitro titration method in a method for the in vitro evaluation and/or monitoring of the effectiveness of a biological production or treatment method or of one or more steps in such a method for the elimination of an NCTA, and to the application thereof in a method for the in vitro evaluation and/or monitoring of a decontamination procedure.

REFERENCES:
patent: 6605445 (2003-08-01), Petteway et al.
Vilette et al. (PNAS, Mar. 2001, 98(7), pp. 4055-4059).
Vilette, D. et al., “Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein” Proceedings of the National Academy of Sciences, vol. 98, No. 7, pp. 4055-4059, Mar. 27, 2001.
Sabuncu, Elifsu et al., “PrP polymorphisms tightly control sheep prion replication in cultured cells” Journal of Virology, vol. 77, No. 4, pp. 2696-2700, Feb. 2003.
Laude, H. et al., “New in vivo and ex vivo models for the experimental study of sheep scrapie: development and perspectives” Comptes Rendus—Biologies, vol. 325, No. 1, pp. 49-57, Jan. 2002.
Stenland, Christopher J. et al., “Partitioning of human and sheep forms of the pathogenic prion protein during the purification of therapeutic proteins from human plasma” Transfusion, vol. 42, No. 11, pp. 1497-1500, Nov. 2002.
Vey, M. et al., “Purity of spiking agent affects partitioning of prions in plasma protein purification”, Biologicals, vol. 30, No. 3, pp. 187-196, Sep. 2002.
Lau, W. et al., “Polymerase chain reaction based assessment of leukoreduction efficacy using a cytomegalovirus DNA transfected human T-cell line” Journal of Clinical Virology, vol. 11, No. 2, pp. 109-116, Aug. 20, 1998.
MacGregor, I. “Prion protein and developments in its detection” Transfusion Medicine, vol. 11, No. 1, pp. 3-14, Feb. 2001.
Archer, Fabienne et al., “Cultured peripheral neuroglial cells are highly permissive to sheep prion infection” Journal of Virology, vol. 78, No. 1, pp. 482-490, Jan. 2004.
Vilette D. et al., “Ex vivo propagation of Infectious sheep scrapie agent In heterologous epithelial cells expressing ovine prion protien,” Proceedings of the National Academy of Sciences of the United States, vol. 98, No. 7, Mar. 27, 2001, pp. 4055-4259.
Sabuncu, Elifsu et al., “PrP polymorphisms tightly control sheep prion replication in cultured cells,” Journal of Virology, vol. 77. No. 4, Feb. 2003, pp. 2696-2700.
Laude, H. et al., “New in vivo and ex vivo models for the experiemental study of sheep scrapie: development and perspectives,” Comptes Rendeus—Biolgies, Elsevier, Paris, FR, vol. 325, No. 1, Jan. 2002, pp. 49-57.
Archer, Fabienne et al., “Cultured peripheral neuroglial cells are highly permissive to sheep prion infection,” Journal of Virology, vol. 78, No. 1, Jan. 2004, pp. 482-490.
Stenland, Christopher et al., “Partitioning of human and sheep forms of the pathogenic prion protein during the purification of therapeutic proteins from human plasma,” Transfusion (Bethesda), vol. 42, No. 11, Nov. 2002, pp. 1497-1500.
Vey, M. et al., “Purity of spiking agent affects partitioning of prions in plasma protein purification,” Biologicals, vol. 30, No. 3, Sep. 2002, pp. 187-196.
Lau, Wendy et al. “Polymerase chain reaction based assessment of leukoreduction efficacy using a cytomegalovirus DNA transfected human T-cell line,” Journal of Clinical Virology: The Official Publication of the Pan American Society for Clinical Virology, Netherlands, vol. 11, No. 2, Aug. 20, 1998, pp. 109-116.
MacGregor I., “Prion protein and developments in its detection,” Transfusion Medicine, Oxford, GB, vol. 11, No. 1, Feb. 2001, pp. 3-14.

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