Method for the in vitro propagation and maintenance of cells

Details Method for the in vitro propagation and maintenance of cells Method for the in vitro propagation and maintenance of cells

1973-07-02

1976-12-14

Rosen, Sam

Textiles: fiber preparation

C12K 900

Patent

active

039973964

ABSTRACT:
The propagation and/or maintenance of living cells in vitro is accomplished by attaching and growing cells on one side or surface of a hollow fiber membrane. The attached cells are propagated and/or maintained by passing oxygen through the membrane from the other side and into contact with the cells and simultaneously incubating the cells in a nutrient medium. This method is employed in tissue and cell culturing of altered and unaltered cells which attach to a surface for maintenance and growth.

REFERENCES:
patent: 3027305 (1962-03-01), Freeman
patent: 3821087 (1974-06-01), Knazek et al.
patent: 3873423 (1975-03-01), Munder et al.
Knazek et al.-Science-vol. 178, (Oct.-Dec. 1972), pp. 65 and 66.
Leighton-J. of the U.S. National Cancer Inst., vol. 12, No. 3 (1951), p. 545.

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