Method for the improved production and recovery of poly-.beta.-h

Details Method for the improved production and recovery of poly-.beta.-h Method for the improved production and recovery of poly-.beta.-h

1992-05-29

1996-04-30

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435141, 435170, C12P 2106, C12P 752, C12P 104

Patent

active

055124569

ABSTRACT:
Poly-.beta.-hydroxybutyrate is produced by providing a culture of Escherichia coli bacterial host cells transformed by a DNA sequence coding for the biosynthetic pathway of poly-.beta.-hydroxybutyrate and a DNA sequence coding for the lysozyme gene; growing the culture and obtaining expression of the poly-.beta.-hydroxybutyrate biosynthetic pathway and the lysozyme gene in each Escherichia coli bacterial host cell; lysing the Escherichia coli bacterial host cells and collecting the poly-.beta.-hydroxybutyrate. An Escherichia coli HMS174(p4A BstB!, pLysS) deposited with the American Type Culture Collection under Accession No. 69001, comprising a plasmid containing a biosynthetic pathway coding for poly-.beta.-hydroxybutyrate and a plasmid containing a lysozyme gene is disclosed.

REFERENCES:
Slater et al., "Cloning and Expression in E. coli of the A. eutrophus H16 PHB Biosynth. Pathway", J. Bact. 170(10):4431-4436 (Oct. 1988).
Studier et al., "Use of T7 RNA Polymerase to Direct Expression of Cloned Genes", Meth. Enz. 185:60-89 (1990).

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