Method for the improved determination of white blood cell subpop

Details Method for the improved determination of white blood cell subpop Method for the improved determination of white blood cell subpop

1993-07-22

1994-05-31

Housel, James C.

Chemistry: analytical and immunological testing

Biological cellular material tested

436175, 436176, 436179, 356 39, G01N 128

Patent

active

053169514

ABSTRACT:
A lytic reagent composition highly selective in its interactions with the cell membranes of white blood cells and also provides a method of using the reagent composition in a particle analyzing system to effect a significantly improved white blood cell differential determination. The lytic reagent composition is characterized by an ability to selectively shrink the white blood cells into the increasing size order of lymphocytes, basophils, monocutes, eosinophils and neutrophils, and effect a five component separation of these major subpopulations of white blood cells on the histogram of an automated blood analyzer when used in conjunction with a suitable blood diluent.

REFERENCES:
patent: 4521518 (1983-06-01), Carter et al.
patent: 4529705 (1985-07-01), Larsen
patent: 4745071 (1988-05-01), Lapicola et al.
patent: 4962038 (1990-10-01), Carter et al.

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