Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-02-22
1998-04-07
Knode, Marian C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436518, 436811, G01N 33535, G01N 33569, G01N 33543
Patent
active
057363480
ABSTRACT:
This patent deals with the use of Trypanosoma cruzi antigens obtained using recombinant DNA techniques, or synthetic peptides derived from these antigens, and their use in the immunological diagnosis of Chagas Disease using either ELISA, or western blot, or dot-blot, or hemagglutination, or agglutination, or monoclonal antibodies, or radioimmunoassay. The antigens or synthetic peptides are characterized by displaying the following aminoacid sequences: Ag1: valine (or alanine), alanine, glutamic acid, alanine (orthreonine), glutamic acid, lysine, glutamine, lysine (or arginine), alanine, alanine, glutamic acid, alanine (or serine), threonine (or methionine or alanine) and lysine. Ag2: methionine, glutamic acid, glutamine, glutamic acid arginine, arginine, glutamine, leucine, leucine, glutamic acid, lysine, aspartic acid, proline, arginine, arginine, asparagine, alanine, lysine, glutamic acid, isoleucine alanine, alanine, leucine, glutamic acid, glutamic acid, serine, methionine, asparagine, alanine, arginine, alanine, glutamine, glutamic acid, leucine, alanine, arginine, glutamic acid, lysine, lysine, leucine, arginine, aspartic acid, arginine, alanine, phenylalanine, leucine, aspartic acid, glutamine, lysine, proline, glutamic acid, arginine, valine, proline, leucine, alanine, aspartic acid, valine, proline, leucine, aspartic acid, aspartic acid, aspartic acid, serine, aspartic acid, phenylalanine, valine and alanine.
REFERENCES:
Groeneveld, R.A., "An Introduction to Probability and Statistics Using Basic", Marcel Dekker, Inc., NY., pp. 377-382 (1979).
Krieger et al., "Use of Recombinant Antigens for the Accurate Immunodiagnosis of Chagas'Disease", Am. J. Trop. Med. Hyg. 46(4):427-434 (1992).
A.O. Luquetti, "Use of Trypanosoma cruzi defined proteins for diagnosis-multicentre trial serological and technical aspects", Mem. Inst. Oswaldo Cruz, Rio de Janeiro, vol. 85(4): 497-505, Feb. 7 1991.
A. Moncayo & A.O. Luquetti, "Multicentre double blind study for evluation of Trypanosoma cruzi defined antigens as diagnostic reagents", Mem. Inst. Oswaldo Cruz, Rio de Janeiro, vol. 85(4): 489-495, Feb. 7, 1991.
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Seebeck, et al., "The Cytoskeleton of Trypanosomes", Parasitology Today, vol. 6, No.2, pp. 49-52, 1990.
Enzyme-Immunoassay, Appendix 4, An Example of the Indirect Microplate Elisa, 1980, p. 194.
Krieger et al., Use of recombinant antigens for the immunological diagnosis of Chagas disease, Mem. Inst. Oswaldo Cruz 85 (Suppl. I), 54, 1990.
Harlow et al., Antibodies A Labortory Manual. Cold Spring Harbor Laboratory, 1988. p. 348; p.592.
La faille et al., Structure and expression of two Tryanosoma Crugi genes encoding antigenic proteins bearing repetitive epitopes. Molec and Biochem. Parasitol. 35: 127-136. 1989.
Odell et al., Eds, Principles of Competitive Protein-Binding Assays. John Wiley & Sons, New York, 1971, pp. 243-254.
Cerqueira De Almeida Elza Carmen
Goldenberg Samuel
Krieger Marco Aurelio
Fundacao Oswaldo Cruz (Fiocruz)
Knode Marian C.
Wortman Donna C.
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