Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-09-02
1990-09-04
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435810, 436513, 436548, 436825, 436827, G01N 33535
Patent
active
049544330
ABSTRACT:
A method is disclosed for the immunolocalization of epitopes of non-glucidic nature, in cellular cultures, in tissue slices, or immobilized on such supports as nitrocellulose, by means of the incubation of the sample with specific antibodies for the antigen to be immunolocalized, belonging to the class of the IgM's, and sequential treatment of concanavalin A and with an enzymatic marker capable of binding with concanavalin A, such as, e.g., peroxidase. The method comprises, before the incubation, a suitable treatment of the sample under test, e.g., with periodic acid, in order to prevent concanavalin A from binding with the carbohydrates present in the sample.
REFERENCES:
patent: 4289747 (1981-09-01), Chu
Petracca Roberto
Ruggiero Paolo
Kepplinger Esther M.
Scheiner Laurie A.
Sclavo S.p.A.
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