Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-12-02
1999-02-09
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C12Q 168
Patent
active
058692509
ABSTRACT:
A method for identifying biologically significant peptide-DNA binding interactions and sequence-specific DNA-binding peptides in vivo using combinatorial oligonucleotide libraries is disclosed. Target DNA sequences include promoter sequences, 5' and 3' regulatory sequences, and exon and intron regulatory sequences. Preferably, a yeast combinatorial library is employed. Peptides that bind to specific DNA sequences, identified by the above method, are disclosed. Also disclosed is a method for identifying nontoxic compounds that inhibit gene expression in host cells in vivo.
REFERENCES:
J.J. Li et al., "Isolation of ORC6, a Component of the Yeast Origin Recognition Complex by a One-Hybrid System", Science 262: 1870-1874, Dec. 1993.
C. Inouye et al., "Isolation of a cDNA Encoding a Metal Response Elelment Binding Protein Using a Novel Expression Cloning Procedure: The One Hybrid System", DNA Cell Biol. 13(7): 731-742, Jul. 1994.
M. Yang et al., "Protein-Peptide Interactions Analyzed With the Yeast Two-Hybrid System", Nuc. Acids Res. 23(7): 1152-1156, Mar. 1995.
Cheng Xiaojun
Juliano R. L.
Prouty Rebecca E.
The University of North Carolina at Chapel Hill
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