Method for the identification of gene transcripts with...

Details Method for the identification of gene transcripts with... Method for the identification of gene transcripts with...

2006-09-05

2006-09-05

Martinell, James (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C702S020000

Reexamination Certificate

active

07101665

ABSTRACT:
A method for identification of gene transcripts comprises the steps of: generating a set of raw sequences by sequencing of biological material; isolating ditags from the set of raw sequences; isolating tags from the ditags; determining abundance of the tags; and identifying the tags, the method providing a step of reducing the amount of sequencing errors by using a statistical model for sequencing errors.

REFERENCES:
patent: 5695937 (1997-12-01), Kinzler et al.
patent: 5970500 (1999-10-01), Sabatini et al.
patent: WO 99/37816 (1999-07-01), None
Audic et al., “The Significance of Digital Gene Expression Profiles”,Genome Research, Oct. 1997; 7(10), pp. 986-995
Ewing et al., “Base-Calling of Automated Sequencer Traces UsingPhred. II, Error Probabilities”,Genome Research, Mar. 1998; 8(3), pp. 186-194.
Velculescu et al., “Serial Analysis of Gene Expression”,Science, Oct. 1995; 270, pp. 484-487.
Velculescu, “Tantalizing Transcriptomes—SAGE and Its Use in Global Expression Analysis”,Science, 1999, 286, pp. 1491-1942, obtained from the Internet Jun. 09, 2002 at http://www.sciencemag.org/cgl/content/full/286/5444/1491.

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