Method for the identification of anesthetics

Details Method for the identification of anesthetics Method for the identification of anesthetics

2006-09-26

2006-09-26

Canella, Karen A. (Department: 1643)

Chemistry: molecular biology and microbiology

Condition responsive control process

C435S004000, C436S055000

Reexamination Certificate

active

07112403

ABSTRACT:
Human and mouse TREK-1 potassium transport proteins are disclosed as well a method for using potassium transport proteins for identifying substances having anesthetic properties, such as producing a safe, reversible state of unconsciousness with concurrent amnesia and analgesia in a mammal upon inhalation, in a method comprising (a) contacting the test substance with a mammalian potassium transport protein, wherein said potassium transport protein exhibits outward-going potassium rectification; and (b) determining the potassium transport activity of the potassium transport protein, wherein an activation of potassium transport is indicative of the test substance having anesthetic properties.

REFERENCES:
Burgess et al “Possible Dissociation of the Heparin-binding and Mitogenic Acitivies of Heparin-binding Growth Factor-1 from Its Receptor-binding Activities by Site-directed Mutagenesis of a single Lysine Residue”, Journal of Cellular biology, vol. 111, pp.
Lazar et al, “Transforming Growth Factor Alpha: Mutation of Aspartic Acid 47 and Leuine 48 Results in Different Biological Activities”, Molecular and Cellular Biology, vol. 8, pp. 1247-1252.
Franks and Lieb, Nature, 1988, vol. 333, pp. 662-664.
Patel et al (Nature Neuroscience, 1999, vol. 2, pp. 422-426).
Leonoudakis et al (Journal of Neuroscience, 1998, vol. 18, pp. 868-877).
Bowie et al (Science, 1990, vol. 257, pp. 1306-1310).
Nicoll, R.A. and D.V. Madison (1982) General anesthetics hyperpolarize neurons in the vertebrate central nervous system. Science 217, 1055-1077.
Berg-Johnson, J. and I.A. Langmoen (1987) Isoflurane hyperpolarizes neurons in rat and human cerebral cortex. Acta Physiol. Scand. 130, 679-685.
Felisberti, F., B. Antkowiak and K. Kirschfeld (1997) Effects of Volatile anesthetics on the membrane potential and ion channels of cultured neocortical astrocytes. Brain Res. 766, 56-65.
MacIver, M.B. and J.J. Kendig (1991) Anesthetic effects on resting membrane potential are voltage-dependent and agent specific. Anesthesiology 74, 83-88.
Franks, N.P. and W.R. Lieb (1988) Volatile general anesthetics activate a novel neuronal K+ current. Nature 333, 662-664.
Franks, N.P. and W.R. Lieb (1991) Stereospecific effects of inhalational general anesthetic optical isomers on nerve ion channels. Science 254, 427-430.
Winegar, B.D., D.F. Owen, C.S. Yost, J.R. Forsayeth, and E. Mayeri (1996) Volatile general anesthetics produce hyperpolarization of Aplysia neurons by activation of a discrete population of baseline potassium channels. Anesthesiology 85, 889-900.
Fink, M. et al. (1996) Cloning, functional expression and localization of a novel unconventional outward rectifier K+ channel. EMBO J. 15, 6854-6862.
Duprat, F. et al (1997) TASK, a human background K+ channel to sense external pH variations near physiological pH. EMBO J. 16, 5464-5471.
Franks, N.P. and W.R. Lieb (1994) Molecular and cellular mechanisms of general anaesthesia. Nature 367, 607-614.
Fink, M. et al. (1998) A neuronoal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids. EMBO J. 17, 3297-3308.
Lesage, F et al (1996) TWIK-1, a ubiquitous human weekly inward rectifying K+ channel with a novel structure. EMBO J. 15, 1004-1011.
Lesage F. et al. (1996) Dimerization of TWIK-1 K+ channel subunits via a disulfide bridge. EMBO J. 15, 6400-6407.
Patel, A. et al (1998) A mammalian two pore domain mechano-gated S-type K+channel. EMBO J. 17, 4283-4290.
Mihic, S.J. et al. (1997) Sites of alcohol and volatile anaesthetic action on GAGA(A) and glycine receptors. Nature 389, 385-389.
Harris, R.A., S.J. Mihic, J.E. Dildy-Mayfied and T.K. Machu (1995) Actions of anesthetics on ligand-gated ion channels: role of receptor subunit composition. Faseb J. 9, 1454-1462.
Yon, J. and M. Fried (1989) Precise gene fusion by PCR. Nucleic Acids Res. 17, 4895.

Affiliated with

Also associated with

Rating

Method for the identification of anesthetics does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the identification of anesthetics, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the identification of anesthetics will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3571502