Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-06-14
2005-06-14
Marschel, Ardin H. (Department: 1631)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S004000, C435S029000, C435S069100, C435S440000
Reexamination Certificate
active
06905818
ABSTRACT:
The present invention relates to an improved method for the identification and optionally the characterisation of interacting molecules designed to detect positive clones from the rather large numbers of false positive clones isolated by conventional two-hybrid systems. The method of the invention relies on a novel combination of selection steps used to detect clones that express interacting molecules from false positive clones. The present invention provides for high-throughput interaction screens for the reliable identification of interacting molecules, which in turn can lead to the identification of substances inhibiting said interactions. Such inhibitors can find their use in the formulation of a pharmaceutical composition. The present invention further relates to kits useful for carrying out the method of the invention.
REFERENCES:
patent: 4657858 (1987-04-01), Davison
patent: 4981783 (1991-01-01), Augenlicht
patent: 5283173 (1994-02-01), Fields et al.
patent: 5569588 (1996-10-01), Ashby et al.
patent: 0 790 304 (1997-08-01), None
patent: WO 96/32503 (1996-10-01), None
patent: WO 97/23609 (1997-07-01), None
patent: WO 97/31112 (1997-08-01), None
patent: WO 97/47763 (1997-12-01), None
patent: WO 98/07845 (1998-02-01), None
Arkin et al., Bio/Technology, vol. 8, pp. 746-749 (1990).
Yang et al., Bio/Technology, vol. 6, pp. 939-942 (1988).
Brent et al., “Understanding Gene and Allele Function with Two-Hybrid Methods,” Annual Review of Genetics, vol. 31, pp. 663-704 (1997).
Tsukamoto, Y. et al. Effects of Mutations of RAD50, RAD51, RAD52, and Related Genes on Illegitimate Recombination inSaccharomyces cerevisiae. Genetics142, 383-391 (Feb. 1996).
Allen, J. et al., “Finding Prospective Partners in the Library: The Two-hybrid System and Phage Display find a Match”, TIBS 20:511-516 (1995).
Anderson, M. et al., “Simultaneous Fluorescence-Activated Cell Sorter Analysis of Two Distinct Transcriptional Elements Within a Single Cell Using Engineered Green Fluorescent Proteins”, Proc. Natl. Acad. Sci. USA 93: 8508-8511 (Aug. 1996).
Barillot, E. et al., “Theoretical Analysis of Library Screening Using a N-Dimensional Pooling Strategy”, Nucleic Acids Research 19(22):6241-6247 (1991).
Bartel, P. et al., “Elimination of False Positive That Arise in Using the Two-Hybrid System”, BioTechniques 14(6): 920-924 (1993).
Bartel, P. et al., “A Protein Linkage Map ofEscherichia coliBacteriophage T7”, Nature Genetics 12: 72-77 (Jan. 1996).
Bendixen, C. et al., “A Yeast Mating-Selection Scheme for Detection of Protein-Protein Interactions”, Nucleic Acids Research 22(9): 1778-1779 (1994).
Benton, David, “Bioinformatics—Principles and Potential of a New Multidisciplinary Tool”, Trends in Biotechnology 14: 261-272 (1996).
Boeke, J. et al., “A Positive Selection for Mutants Lacking Orotidine-5′-phosphate Decarboxylase Activity in Yeast: 5-fluoro-orotic Acid Resistance”, Gen. Genet. 197: 345-346 (1984).
Breeden, L. and Nasmyth, K., “Regulation of the Yeast HO Gene”, Cold Spring Harbor Symp. Quant. Biol. 50: 643-650 (1985).
Chen, Claudia and Okayama, H., “High-Efficiency Transformation of Mammalian Cells by Plasmid DNA”, Molecular and Cellular Biology 7(8): 2745-2752 (Aug. 1987).
Cubitt, A. et al., “Understanding, Improving and Using Green Fluoresecnt Proteins”, TIBS20:448-455 (Nov. 1995).
Dang, C. et al., “Intracellular Leucine Zipper Interactions Suggest c-Myc Hetero-Oligomerization”, Molecular and Cellular Biology 11(2): 654-962 (Feb. 1991).
DeRisi, J. et al., “Use of a cDNA Microarray to Analyse Gene Expression Patterns in Human Cancer”, Nature Genetics 14: 457-460 (1996).
Dove, S. et al., “Activation of Prokaryotic Transcription Through Arbitrary Protein-Protein Contacts”, Nature 386: 627-629 (Apr. 10, 1997).
Drmanac et al.; “Sequencing of Megabase Plus DNA by Hybridization: Theory of the Method”, Genomics 4 : 114-128 (1989).
Fearon, E. et al., “Karoplasmic Interaction Selection Strategy: A General Strategy to Detect Protein-Protein Interactions in Mammalian Cells”, Proc. Natl. cad. Sci. USA 89: 7958-7962 (Sep. 1992).
Feinberg, A. and Vogelstein, B., “A technique for Radiolabeling DNA Restriction Endonuclease Fragments to High Specific Activity”, Analytical Biochemistry 132:6-13 (1983).
Fields, Stanley and Song, O., “A Novel Genetic System to Detect Protein-Protein Interactions”, Nature 340: 245-246 (Jul. 20, 1989).
Fleischmann, R. et al., “Whole-Genome Random Sequencing and Assembly ofHaemophilus influenzaeRD”, Science 269: 496-512 (Jul. 28, 1995).
Gietz, A. et al., “Improved Method for High Efficiency Transformation of Intact Yeast Cells”, Nucleic Acids Research 20(6): 1425 (1992).
Gossen, Manfred and Bujard, H., “Tight Control of Gene Expression in Mammalian Cells By Tetracyclin-Responsive Promoters”, Proc. Natl. Acad. Sci. USA 89: 5547-5551 (Jun. 1992).
Gossen, M. et al., “Transcriptional Activation by Tetracyclines in Mammalian cells”, Science 268: 1766-1769 (Jun. 23, 1995).
Graham. F. L., and Smiley, J., “Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5”, J. Gen. Virol. 36:59-72 (1977).
Graham, F. L. and Van der Eb, A. J., “Transformation of Rat Cells by DNA of Human Adenovirus 5”, Virology 54: 5 6-539 (1973).
Gress, T. M. et al., “A Pancreatic Cancer-Specific Expression Profile”, Oncogene 13: 1819-1830 (1996).
Guzman, L-M et al., “Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBAD Promoter”, J Bacteriology 177(14): 4121-4130 (Jul. 1995).
Han, Limin and Colicelli, J., “A Human Protein Selected for Interference with Ras Function Interacts Directly with Ras and Completes with Rafl”, Molecular and Cellular Biology 15(3): 1318-1323 (Mar. 1995).
Harper J. W. et al., “The p21 Cdk-Interacting Protein Cip1 is a Potent Inhibitor of G1 Cyclin-Dependent Kinases”, Cell 75: 805-816 (Nov. 19, 1993).
Hoffmann, Werner, “Molecular Characterization of the CAN1 Locus inSaccharomyces cerevisiae”, J. Biological Chemistry 260(21): 11831-11837 (1985).
Hoheisel, J. et al., “Use of High Coverage Reference Libraries ofDrosophila melanogasterfor Relational Data Analysis”, Mol. Biol. 220:903-914 (1991)
Johnston F. R. et al.; “Autoradiography Using Storage Phosphor Technology”, Electrophoresis, 11(5): 353-450 (May 1990).
Kaufer, N. et al., “Cycloheximide Resistance in Yeast: The Gene and Its Protein”, Nucleic Acids Research 11(10): 31233135 (Nov. 10, 1983).
Kawaguchi, Y. et al., “CAG Expansions in a Novel Gene for Machado-Joseph Disease at Chromosome 14q32.1”, Nature Genetics 8: 221-228 (Nov. 1994).
Li, Joachim J. and Herskowitz, I., “Isolation of ORC6, a Component of the Yeast Origin Recognition Complex by a One-Hybrid System”, Science 262: 1870-1874 (Dec. 17, 1993).
Larin, Zoia and Lehrach, H., “Yeast Artificial Chromosomes: An Alternative Approach to the Molecular Analysis of Mouse Developmental Mutations”, Genet. Res. 56: 203-208 (1990).
Le Douarin, B. et al., “A New Version of the Two-Hybrid Assay for Detection of Protein-Protein Interactions”, Nucleic Acids Research 23(5): 876-878 (1995).
Link, A. et al., “Methods for Generating Precise Deletions and Insertions in the Genome of Wild-typeEscerichia coli:Application to Open Reading Frame Characterization”, J. Bacteriology 179(20): 6228-6237 (Oct. 1997).
Liu, J. et al., “Large-Scale Cloning of Human Chromosome 2-Specific Yeast Articicial Chromosomes (YACs) Using and Interspersed Repetitive Sequences (IRS)-PCR Approach”, Genomics 26: 178-191 (1995).
Meier-Ewert, S. et al., “An Automated Approach to Generating Expressed Sequence Catalog
Bancroft David
Lehrach Hans
Maier Elmar
Meier-Ewert Sebastian
Wanker Erich
Marschel Ardin H.
Max-Planck-Gesellschaft zur Forderungder Wissenschaften
Ropes & Gray LLP
LandOfFree
Method for the identification and characterization of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the identification and characterization of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the identification and characterization of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3519431