Method for the genetic detection of microorganisms

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C12K 104

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039309568

ABSTRACT:
A method for the genetic detection of specific microorganisms based on the phenomenon of transformation is disclosed. Variants such as auxotrophs which are distinguishable from their wild, parent strain are transformed by deoxyribonucleic acid (DNA) extracted from microorganism cells present in a biological test sample. The detection of transformed cells indicates the presence of the specific microorganism under determination in the sample. The transforming-DNA is in a sterile, crude form, thereby providing a simple, rapid, and highly specific test method.

REFERENCES:
J. Gen. Microbiol., (1969), 57, pp. 125-130.
J. Gen. Microbiol., (1961), 26, pp. 303-312.

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