Method for the extraction of periplasmic proteins from prokaryot

Details Method for the extraction of periplasmic proteins from prokaryot Method for the extraction of periplasmic proteins from prokaryot

1996-01-31

1997-12-23

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 694, 435 6952, 4352523, C12N 120, C12N 121, C12P 2100

Patent

active

057006652

ABSTRACT:
The invention relates to a method for the extraction of recombinant periplasmic proteins wherein arginine is used as the extraction agent. In particular, the invention relates to a method for the extraction of a periplasmic protein of interest, which essentially consists in:

REFERENCES:
Patent Abstracts of Japan, vol. 11 No. 246 (C-439), Aug. 11, 1987 & JP-A-62 051983 (Yoshihide Hagiwara) Mar. 6, 1987.
Patent Abstracts of Japan, vol. 11 No. 232 (C-437), 29 Juillet 1987 & JP-A-62 044198 (Hanaka Keizo) Feb. 26, 1987.
Dissertation Abstracts International B, vol. 37, No. 5, 1976 pp. 200-201, Brown et al. "Purification by Bioaffinity Chromatography of Two Arginine-Specific Periplasmic Binding Proteins".

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