Combinatorial chemistry technology: method – library – apparatus – Method of creating a library – Biochemical method
Reexamination Certificate
2011-03-22
2011-03-22
Liu, Sue (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Method of creating a library
Biochemical method
C435S006120
Reexamination Certificate
active
07910522
ABSTRACT:
The present invention relates to methods for the identification or cloning of polynucleotides encoding a selected phenotype, particularly from environmental DNA. In a specific embodiment, the method comprises (i) cloning environmental DNA fragments intoE. colicloning vectors to produce a metagenomic library, (ii) identifying or selecting cloning vectors in the library which contain DNA fragments having a particular characteristic of interest, (iii) modifying the identified or selected cloning vectors into shuttle or expression vectors for transfer and integration in a selected host cell, (iv) transferring the modified cloning vectors into the selected host-cell and (v) identifying or cloning the DNA fragments contained in the modified cloning vectors which encode the selected phenotype in the selected host cell.
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Gillet Benjamin
Nalin Renaud
Pujic Petar
Tuphile Karine
Libragen
Liu Sue
Saliwanchik Lloyd & Eisenschenk
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