Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1993-12-10
1995-08-01
Ziska, Suzanne E.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43524021, 4352401, C12N 500
Patent
active
054379946
ABSTRACT:
Methods, including culture media conditions, which provide for ex vivo human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
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"Retrovirus-mediated transfer and expression of drug resistance genes
Clarke Michael F.
Emerson Stephen G.
Palsson Bernhard O.
Regents of the University of Michigan
Ziska Suzanne E.
LandOfFree
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