Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-10-16
1993-03-23
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435 30, 435 31, 435 33, C12Q 102, C12Q 124, C12Q 122, C12Q 118
Patent
active
051963132
ABSTRACT:
A method for the rapid and reliable in vitro determination of the anticoccidial activity of chemotherapeutic agents. The invention involves incubating free-living sporozoites in a nutrient medium in the presence of the anticoccidial agent being assessed and 2,3,5-triphenyltetrazolium chloride. Living sporozoites reduce the above compound to the water-insoluble vivid-red formazan pigment but will not stain with trypan blue; dead sporozoites do not form this red pigment but will stain with trypan blue.
REFERENCES:
patent: 4199515 (1980-04-01), Yamagishi et al.
patent: 4882346 (1989-11-01), Driscoll et al.
Mason et al (1968) Exp. Parasitol 23:212-220.
James (1980) Parasitology 80:301-312.
Millard et al (1974) Abstract 166237e Chemical Abstracts.
Weathersby et al (1981) Abstract 33948 Biol Abst 73(5).
Koopman et al (Jul. 1986) Toxicity Screening-in Wastewater Systems. In: Toxicity Testing Using Microorganisms CRC Press, Boca Raton, Fla. pp. 123-125.
Bitton (1983) CRC 13(1) 57-67.
American Cyanamid Company
Climenson Peggy A.
Nucker Christine M.
Preston D. R.
LandOfFree
Method for the evaluation of anticoccidial activity of compounds does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the evaluation of anticoccidial activity of compounds, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the evaluation of anticoccidial activity of compounds will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1350923